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The effect of the autouse pragma is to reduce the loading and compilation time for a script and also to reduce the number of modules that are loaded at startup in scripts where specific external functions are used only in certain execution trees Unfortunately, the pragma also has the effect of delaying the loading of the module until execution time, thus bypassing the usual checks that are made when importing a module and shifting the responsibility of reporting any errors to the execution stage, rather than the compilation stages Problems can occur when there are bugs or typographical errors in the modules being autoused, because your program will not fall over when the module is imported, but when a function is actually used Additionally, any module that relies on early initialization (say, within a BEGIN {} block) may fail because it expects the initialization to occur during the initial compilation of the whole script You can get around the bugs in autouse modules during the development phase by placing a genuine use statement for the autouse modules For example,

Figure 5-7

use File::Basename use autouse 'File::Basename' => qw(basename dirname);

The first line masks the second; when you come to release your script and/or module to the public, just comment out the first line and let the autouse pragma do its work

The base pragma establishes a relationship with a base class at compile (rather than execution) time In effect, this operation is equal to adding the specified classes to the @ISA array during the module initialization, such that

REGULATION OF INFORMATION FLOW from DNA to RNA to protein Every aspect of the process is controlled, and alternatives are available that affect which information is expressed at what time

package Object; use base qw(Foo Bar);

BEGIN { require Foo; require Bar; push @ISA, qw(Foo Bar); }

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Inducible or repressible refers to the type of response the system makes to the presence of a metabolite Inducible genes are turned on when they sense the presence of a metabolite Usually, this means that the metabolite is a precursor of something the cell needs If the precusor is present, inducible genes are turned on to metabolize it Repressible genes are turned off by the presence of a metabolite These genes are usually involved in the synthesis of the metabolite If the cell has enough of the metabolite, the pathway is turned off (repressed) If the metabolite is not present, the pathway is turned on Operons are clusters of genes located next to each other The proteins they make are usually required at the same time and for the same overall function The transcription of genes in an operon is regulated by a common regulatory site(s) on the DNA Inducible or repressible operons may be created by either positive or negative regulatory elements The concepts of inducible/repressible and positive/negative control are related but independent There are then two possibilities for regulation of inducible pathways If a regulatory protein binds to DNA when it senses the metabolite and then activates transcription, this is a positive way of inducing RNA synthesis An inducible gene can also function by negative regulation If a regulatory protein binds to DNA and shuts off transcription when the metabolite is absent, and the protein is released from the DNA when it binds the metabolite, the net effect is the same (increased transcription) Inducible genes can be regulated by either positive or negative effectors There are also two ways to have repressible genes using positive and negative regulation

Because of this, any base class not yet loaded but explicitly listed to the base pragma will be loaded automatically via require Note, however, that the normal inheritance rules are not followed and the %INC hash is not used to determine whether a base class has already been loaded This is because use of base could cause Perl to enter a cycle where it tries to load a class from a file that has already been imported with require Instead, base sets the value of $VERSION in the base class to the string 1, defined by basepm Note that in addition to setting the inheritance, the base module also causes any special field attributes for the classes named to be initialized, providing they use the fields pragma to set the attributes See the entry for fields later in this chapter

The blib pragma forces Perl to look for modules in the blib directory structure, as created by the MakeMaker module This feature is especially useful for testing modules before they are finally installed when using ExtUtils::MakeMaker (see 25 for more information) Ideally, it should be used only on the command line with the -M option:

If dir is specified, it looks in that directory (and subdirectories) for the blib structure If you do not specify dir, it assumes you want to include the current directory You can also use

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